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BQuat Siphoviridae Bacteriophage Genome Annotation and Analysis

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CCURI Fall 2017 Colloquium, Austin, Texas


In 2010, mycobacteriophage BQuat was directly isolated from soil collected on the campus of Washington University in St. Louis, Missouri. BQuat was identified to be a unique Siphoviridae that infects Mycobacterium smegmatis (mc2155). DNA extraction was performed and the DNA was sent to the McDonnell Genome Institute at Washington University for sequencing, making it ready for annotation. BQuat’s genome is 41893 bp in length and is a member of the G1 Cluster. Through analysis of BQuat’s genome, we assigned putative functions to BQuat proteins utilizing bioinformatic resources. BQuat’s bacterial host M. smegmatis (mc2155) is genetically similar to Mycobacterium tuberculosis. The goal of analyzing BQuat’s genome is to identify genes that likely contribute to viral fitness through interaction with described proteins of M. smegmatis (mc2155), and in turn, M. tuberculosis. Identification of these proteins will progress the aim of finding alternative treatments for humans infected with tuberculosis, as well as adding to the growing knowledge of viral and bacterial interactions and the relationships between the two pathogens.