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Using Your Noodle to Analyze a Branch in the Phamily Tree Genomic Annotation and Analysis of the C1 Myovirus NoodleTree

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SEA Phages Annual Symposium, June, 2017


The Mycobacteriophage, NoodleTree, was discovered in 2016 from soil near the main fountain on the Spring Creek Campus of Collin College in Plano, TX. After isolation, amplification and imaging, it was found to be a unique Myoviridae bacteriophage that infects Mycobacterium smegmatis (mc2155). After DNA extraction, the DNA was sequenced by a team at the Pittsburgh Bacteriophage Institute, and genomic data was made available for annotation. The phage genome has a length of over 150 kbp and is a member of the C1 phage cluster. Upon analysis of the phage genome using DNA Master and its accompanying suite of verifiers, NoodleTree was found to have unique characteristics among viruses that infect M. smegmatis. During annotation, we identified several different genes and gene gaps with unknown/novel function.