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AC2 Poster Session

The Mycobacteriophage, NoodleTree, was discovered in 2016 from soil near the main fountain on the Spring Creek Campus of Collin College in Plano, TX. After isolation, amplification and imaging, it was found to be a unique Myoviridae bacteriophage that infects Mycobacterium smegmatis (mc2155). After DNA extraction, the DNA was sequenced by a team at the Pittsburgh Bacteriophage Institute, and genomic data was made available for annotation. The phage genome has a length of over 150 kbp and is a member of the C1 phage cluster. Upon analysis of the phage genome using DNA Master and its ... Read more

In 2010, mycobacteriophage BQuat was directly isolated from soil collected on the campus of Washington University in St. Louis, Missouri. BQuat was identified to be a unique Siphoviridae that infects Mycobacterium smegmatis (mc2155). DNA extraction was performed and the DNA was sent to the McDonnell Genome Institute at Washington University for sequencing, making it ready for annotation. BQuat’s genome is 41893 bp in length and is a member of the G1 Cluster. Through analysis of BQuat’s genome, we assigned putative functions to BQuat proteins utilizing bioinformatic resources. BQuat’s ... Read more

Soil is one of the richest sources for microbial diversity and one of the most challenging of environments from which to successfully culture and study the microbes present. From an agricultural standpoint, soil microbes carry out a number of essential biochemical reactions necessary for plant survival, including nitrogen fixation and phosphorous solubilization. All of the necessary components to support plant life are present in Martian soil; however, they are not present in sufficient quantities to support plant life without heavy supplementation with fertilizers. Using metagenomic ... Read more

Terpenoids are a diverse class of plant natural products that function as plant hormones, act in plant defense responses and provide structural support to membranes. In addition, terpenoids are commercially valued as essential oils, flavors, fragrances and pharmaceutical drugs. Furano-terpenes isolated from Ipomoea batatas are produced in response to fungal infection. Because of their anti-fungal activities, these terpenes may be particularly useful as new anti-fungal treatments. We are interested in identifying genes involved in the biosynthesis of these compounds. We are currently ... Read more

Wolbachia is a widely-distributed, obligate, intracellular parasite belonging to the alpha-proteobacteria group, sharing features with Rickettsiae-like microorganisms. It is a bacterial endosymbiont whose presence can influence the sex ratio of many arthropod and nematode offspring by a number of different mechanisms. Its impacts on bio-control and speciation have made the study of Wolbachia invaluable. In order to gain insight into the potential impact of this host-parasite interaction on changes in select populations in Kentucky, we set out to characterize a sampling of Wolbachia strains ... Read more

Todd M. Smith [1,2], Sandra Porter [1,2], Linnea Fletcher [2,3]

1. Digital World Biology (DWB), 2. Bio-Link, 3. Austin Community College (ACC)

Students graduating from college in the 21st century need to be skilled in using a computer. Through several experiences including Austin Community College’s (ACC’s) interactions with industry, DWB’s experience, and combined participation in efforts such as the Network for Integrating Bioinformatics into Life Sciences Education (NIBLSE), it is clear students need to be more aware of how computing is used in the life sciences and the ... Read more